In vitro capabilities

CXR has extensive in-house in vitro capabilities and expertise, including experience of preparing, culturing and analysing primary cell lines (e.g. hepatocytes, keratinocytes) and tissue slices. 

This is supported by a full suite of modern laboratory equipment (including two Sciex 6500 triple-quad mass spectrometers, two further triple-quad mass spectrometers, a Xevo G2-XS QTOF/TOF plus a further TOF mass spectrometer, GC, GC-MS, HPLC, advanced microscopy and image analysis, a liquid scintillation counter, Quantitative PCR, full capability in Agilent gene analysis and Ingenuity bioinformatics).

CXR’s in vitro capabilities include:

Permeability and protein binding:

  • Everted intestinal sacs
  • Caco2 / MDCK cell lines
  • Protein binding

Metabolism and drug-drug interactions

  • Microsomes (liver, gut, skin…) – rates and pathways in multiple species (metabolite identification, cold or labelled)
  • Hepatocytes – rates and pathways in multiple species (metabolite identification, cold or labelled)
  • Recombinant enzymes – rates and pathways (metabolite identification, cold or labelled)
  • Reaction phenotyping
  • Enzyme induction in human, mouse, rat hepatocytes (DNA, RNA, protein, activity)
  • CYP inhibition

Primary cell / cell line / tissue slice / protein based assays

  • Microarray Expression Profiling
  • Microarray MicroRNA Profiling
  • Microarray Methylation Profiling
  • RT-PCR
  • Deep Sequencing (outsource)