Technologies

Preclinical models

CXR have developed innovative preclinical screening models to improve lead compound selection by providing a better understanding of metabolism and toxicity.

• The HRN™ mouse model, that lacks hepatic P450 metabolism more....
• Humanised mouse models. CXR have replaced murine PXR and CAR by human genes, and these humanised reflect known species differences in drug disposition and toxicitymore....
• Engineered Biomarker mouse models, containing engineered biomarkers that report on mechanism of toxicity more....

Mechanistic assays and screens

Leveraging a combination of skills in conventional pre-clinical development and state-of-the-art molecular biology, CXR Biosciences have developed a range of mechanistic assays (examples below) and can rapidly develop bespoke assays and screens tailored to the client's requirements more....

• Transactivation assays; cell based transactivation assays that report on the interaction of compounds with nuclear hormone receptors from a variety of species more.... 
• Engineered Biomarker cell lines; cell-based reporter systems that report on specific mechanisms of toxicity of chemicals and drugs more....

Transcription Profiling more....

Transcription profiling measures the changes in gene expression as a result of exposure to compounds or chemicals. CXR Biosciences employs transcription profiling to provide a detailed understanding of the cellular responses to compounds. This is useful in a variety of ADMET applications, but especially in Investigative Toxicology where it is thoughtfully applied to generate hypotheses about the mechanism of toxicity.

Bioinformatic Pathways Analysis

Central to our mechanism based approach is a detailed and thorough understanding of the biochemical pathways involved in the biological response under investigation ( eg. a particular mechanism of action or toxicity of a compound). CXR uses Ingenuity Pathway Analysis bioinformatics software (IPA) to generate hypotheses and identify potential mechanisms that may be involved in the biological actions of compounds or drugs. This is especially useful in elucidating the mechanisms of toxicity in investigative toxicology. more....

ADME (Absorption, Distribution, Metabolism, Elimination) and Toxicology more....

CXR Biosciences offers a comprehensive range of ADME and toxicity screens including animal and human hepatocyte studies, human microsomes, cassette inhibition assays, drug transporter assays, and pre-development in vivo toxicological studies, which are analysed using a state of the art tandem liquid chromatography mass spectrometer (LC/MS/MS).